Role of basic fibroblast growth factor in epidermal stem cells differentiating into neural stem cells in rats / 中华创伤杂志

ABSTRACT

Objective To investigate the effect of basic fibroblast growth factor (bFGF) on the differentiation of epidermal stem cells(ESCs) into nerve cells in rats.Methods The epidermal basal layer tissue of newborn SD rats (1-3 days) were isolated and obtained.ESCs were digested and isolated by rapid attachment to a substrate,and the morphology of ESCs was observed under an inverted microscope.ESCs were cuhured with Keratinocytes serum-free medium (K-SFM).The ESCs were grouped and treated according to the density including Group A0.1 × 107/ml,Group B0.3 × 107/ml.Group C0.5 × 1 07/ml,Group D0.1 × 106/ml,and each group was added bFGF (20 ng/ml).The changes of cell morphology were observed and counted for seven days.The changes of cell markers Nestin and NSE were detected by immunohistochenistry.Results The ESCs of SD rat were isolated successfully.After bFGF induction,the numbers of cells with morphological changes in Groups A and B were larger than those in other two groups in the first 6 days (P ＜ 0.05),and the number in Group A was the largest on the seventh day (P ＜ 0.05).On the third day,Group C had the largest number of cells with changes based on the comparison within the group.There was no change in cell morphology in Group D.Immunohistochemistry showed positive Nestin and NSE.Conclusion The bFGF helps induce the differentiation of ESCs into nerve cells,which is associated with the cell density.