Find and replace: editing human genome in pluripotent stem cells
Protein & Cell
;
(12): 950-956, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-757030
ABSTRACT
Genetic manipulation of human pluripotent stem cells (hPSCs) provides a powerful tool for modeling diseases and developing future medicine. Recently a number of independent genome-editing techniques were developed, including plasmid, bacterial artificial chromosome, adeno-associated virus vector, zinc finger nuclease, transcription activator-like effecter nuclease, and helper-dependent adenoviral vector. Gene editing has been successfully employed in different aspects of stem cell research such as gene correction, mutation knock-in, and establishment of reporter cell lines (Raya et al., 2009; Howden et al., 2011; Li et al., 2011; Liu et al., 2011b; Papapetrou et al., 2011; Sebastiano et al., 2011; Soldner et al., 2011; Zou et al., 2011a). These techniques combined with the utility of hPSCs will significantly influence the area of regenerative medicine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Engenharia Genética
/
Genoma Humano
/
Linhagem Celular
/
Mutagênese Insercional
/
Dedos de Zinco
/
Dependovirus
/
Marcação de Genes
/
Cromossomos Artificiais Bacterianos
/
Biologia Celular
Limite:
Humanos
Idioma:
Inglês
Revista:
Protein & Cell
Ano de publicação:
2011
Tipo de documento:
Artigo
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