Your browser doesn't support javascript.
loading
Targeted deletion of mouse Rad1 leads to deficient cellular DNA damage responses
Protein & Cell ; (12): 410-422, 2011.
Artigo em Inglês | WPRIM | ID: wpr-757086
ABSTRACT
The Rad1 gene is evolutionarily conserved from yeast to human. The fission yeast Schizosaccharomyces pombe Rad1 ortholog promotes cell survival against DNA damage and is required for G(2)/M checkpoint activation. In this study, mouse embryonic stem (ES) cells with a targeted deletion of Mrad1, the mouse ortholog of this gene, were created to evaluate its function in mammalian cells. Mrad1 (-/-) ES cells were highly sensitive to ultraviolet-light (UV light), hydroxyurea (HU) and gamma rays, and were defective in G(2)/M as well as S/M checkpoints. These data indicate that Mrad1 is required for repairing DNA lesions induced by UV-light, HU and gamma rays, and for mediating G(2)/M and S/M checkpoint controls. We further demonstrated that Mrad1 plays an important role in homologous recombination repair (HRR) in ES cells, but a minor HRR role in differentiated mouse cells.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Fisiologia / Raios Ultravioleta / Dano ao DNA / Divisão Celular / Fase G2 / Deleção de Genes / Proliferação de Células / Reparo do DNA / Exonucleases Limite: Animais Idioma: Inglês Revista: Protein & Cell Ano de publicação: 2011 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Fisiologia / Raios Ultravioleta / Dano ao DNA / Divisão Celular / Fase G2 / Deleção de Genes / Proliferação de Células / Reparo do DNA / Exonucleases Limite: Animais Idioma: Inglês Revista: Protein & Cell Ano de publicação: 2011 Tipo de documento: Artigo