Apaf1 inhibition promotes cell recovery from apoptosis
Protein & Cell
;
(12): 833-843, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-757189
ABSTRACT
The protein apoptotic protease activating factor 1 (Apaf1) is the central component of the apoptosome, a multiprotein complex that activates procaspase-9 after cytochrome c release from the mitochondria in the intrinsic pathway of apoptosis. We have developed a vital method that allows fluorescence-activated cell sorting of cells at different stages of the apoptotic pathway and demonstrated that upon pharmacological inhibition of Apaf1, cells recover from doxorubicin- or hypoxia-induced early apoptosis to normal healthy cell. Inhibiting Apaf1 not only prevents procaspase-9 activation but delays massive mitochondrial damage allowing cell recovery.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Células HeLa
/
Doxorrubicina
/
Hipóxia Celular
/
Trifosfato de Adenosina
/
Apoptose
/
Linhagem Celular Tumoral
/
Microscopia Eletrônica de Transmissão
/
Fator Apoptótico 1 Ativador de Proteases
/
Genética
Limite:
Humanos
Idioma:
Inglês
Revista:
Protein & Cell
Ano de publicação:
2015
Tipo de documento:
Artigo
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