Bisindoylmaleimide I enhances osteogenic differentiation
Protein & Cell
;
(12): 311-320, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-757265
ABSTRACT
The Wnt/β-catenin and bone morphogenetic proteins (BMPs) pathways play important roles in controlling osteogenesis. Using a cell-based kinase inhibitor screening assay, we identified the compound bisindoylmaleimide I (BIM) as a potent agonist of the cytosolic β-catenin accumulation in preosteoblast cells. Through suppressing glycogen synthase kinase 3β enzyme activities, BIM upregulated β-catenin responsive transcription and extended duration of BMP initiated signal. Functional analysis revealed that BIM promoted osteoblast differentiation and bone formation. The treatment of human mesenchymal stem cells with BIM promoted osteoblastogenesis. Our findings provide a new strategy to regulate mesenchymal stem cell differentiation by integration of the cellular signaling pathways.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Farmacologia
/
Transdução de Sinais
/
Diferenciação Celular
/
Química
/
Proteínas Morfogenéticas Ósseas
/
Biologia Celular
/
Quinase 3 da Glicogênio Sintase
/
RNA Interferente Pequeno
/
Interferência de RNA
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Protein & Cell
Ano de publicação:
2012
Tipo de documento:
Artigo
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