In vitro assembly of Ebola virus nucleocapsid-like complex expressed in E. coli
Protein & Cell
;
(12): 888-898, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-757381
ABSTRACT
Ebola virus (EBOV) harbors an RNA genome encapsidated by nucleoprotein (NP) along with other viral proteins to form a nucleocapsid complex. Previous Cryo-eletron tomography and biochemical studies have shown the helical structure of EBOV nucleocapsid at nanometer resolution and the first 450 amino-acid of NP (NPΔ451-739) alone is capable of forming a helical nucleocapsid-like complex (NLC). However, the structural basis for NP-NP interaction and the dynamic procedure of the nucleocapsid assembly is yet poorly understood. In this work, we, by using an E. coli expression system, captured a series of images of NPΔ451-739 conformers at different stages of NLC assembly by negative-stain electron microscopy, which allowed us to picture the dynamic procedure of EBOV nucleocapsid assembly. Along with further biochemical studies, we showed the assembly of NLC is salt-sensitive, and also established an indispensible role of RNA in this process. We propose the diverse modes of NLC elongation might be the key determinants shaping the plasticity of EBOV virions. Our findings provide a new model for characterizing the self-oligomerization of viral nucleoproteins and studying the dynamic assembly process of viral nucleocapsid in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
RNA Viral
/
Expressão Gênica
/
Química
/
Montagem de Vírus
/
Nucleocapsídeo
/
Ebolavirus
/
Escherichia coli
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Protein & Cell
Ano de publicação:
2016
Tipo de documento:
Artigo
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