Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes
Protein & Cell
;
(12): 59-68, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-757529
ABSTRACT
With defined culture protocol, human embryonic stem cells (hESCs) are able to generate cardiomyocytes in vitro, therefore providing a great model for human heart development, and holding great potential for cardiac disease therapies. In this study, we successfully generated a highly pure population of human cardiomyocytes (hCMs) (>95% cTnT(+)) from hESC line, which enabled us to identify and characterize an hCM-specific signature, at both the gene expression and DNA methylation levels. Gene functional association network and gene-disease network analyses of these hCM-enriched genes provide new insights into the mechanisms of hCM transcriptional regulation, and stand as an informative and rich resource for investigating cardiac gene functions and disease mechanisms. Moreover, we show that cardiac-structural genes and cardiac-transcription factors have distinct epigenetic mechanisms to regulate their gene expression, providing a better understanding of how the epigenetic machinery coordinates to regulate gene expression in different cell types.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transcrição Gênica
/
Diferenciação Celular
/
Linhagem Celular
/
Regulação da Expressão Gênica
/
Metilação de DNA
/
Perfilação da Expressão Gênica
/
Biologia Celular
/
Miócitos Cardíacos
/
Epigênese Genética
/
Células-Tronco Embrionárias
Tipo de estudo:
Guia de Prática Clínica
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Protein & Cell
Ano de publicação:
2014
Tipo de documento:
Artigo
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