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Microvesicle-delivery miR-150 promotes tumorigenesis by up-regulating VEGF, and the neutralization of miR-150 attenuate tumor development
Protein & Cell ; (12): 932-941, 2013.
Artigo em Inglês | WPRIM | ID: wpr-757549
ABSTRACT
Tumor-associated macrophages (TAMs) mostly exhibit M2-like (alternatively activated) properties and play positive roles in angiogenesis and tumorigenesis. Vascular endothelial growth factor (VEGF) is a key angiogenic factor. During tumor development, TAMs secrete VEGF and other factors to promote angiogenesis; thus, anti-treatment against TAMs and VEGF can repress cancer development, which has been demonstrated in clinical trials and on an experimental level. In the present work, we show that miR-150 is an oncomir because of its promotional effect on VEGF. MiR-150 targets TAMs to up-regulate their secretion of VEGF in vitro. With the utilization of cell-derived vesicles, named microvesicles (MVs), we transferred antisense RNA targeted to miR-150 into mice and found that the neutralization of miR-150 down-regulates miR-150 and VEGF levels in vivo and attenuates angiogenesis. Therefore, we proposed the therapeutic potential of neutralizing miR-150 to treat cancer and demonstrated a novel, natural, microvesicle-based method for the transfer of nucleic acids.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Regulação para Cima / RNA Antissenso / MicroRNAs / Linhagem Celular Tumoral / Fator A de Crescimento do Endotélio Vascular / Exossomos / Células HEK293 / Xenoenxertos / Carcinogênese Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Protein & Cell Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Regulação para Cima / RNA Antissenso / MicroRNAs / Linhagem Celular Tumoral / Fator A de Crescimento do Endotélio Vascular / Exossomos / Células HEK293 / Xenoenxertos / Carcinogênese Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Protein & Cell Ano de publicação: 2013 Tipo de documento: Artigo