MicroRNA-495 induces breast cancer cell migration by targeting JAM-A
Protein & Cell
;
(12): 862-872, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-757649
ABSTRACT
MicroRNAs (miRNAs) are small, non-coding RNAs that function as post-transcriptional regulators of gene expression. The deregulated expression of miRNAs is associated with a variety of diseases, including breast cancer. In the present study, we found that miR-495 was markedly up-regulated in clinical breast cancer samples by quantitative real time-PCR (qRT-PCR). Junctional adhesion molecule A (JAM-A) was predicted to be a potential target of miR-495 by bioinformatics analysis and was subsequently verified by luciferase assay and Western blotting. JAM-A was found to be negatively correlated with the migration of breast cancer cells through loss-of-function and gain-of-function assays, and the inhibition of JAM-A by miR-495 promoted the migration of MCF-7 and MDA-MB-231 cells. Furthermore, overexpression of JAM-A could restore miR-495-induced breast cancer cell migration. Taken together, our findings suggest that miR-495 could facilitate breast cancer progression through the repression of JAM-A, making this miRNA a potential therapeutic target.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Neoplasias da Mama
/
Moléculas de Adesão Celular
/
Regulação Neoplásica da Expressão Gênica
/
Movimento Celular
/
Western Blotting
/
Receptores de Superfície Celular
/
Regiões 3' não Traduzidas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
MicroRNAs
Tipo de estudo:
Estudo prognóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Protein & Cell
Ano de publicação:
2014
Tipo de documento:
Artigo
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