A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer
Journal of Veterinary Science
;
: 260-270, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-758794
ABSTRACT
Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction response regulator (ArsR) and a signal transduction histidine kinase (SthK) were predicted to be located on the same operon. A mutant was constructed by deletion of both of these genes. Duck infection tests showed that genes ArsR and SthK were related to the virulence of the pathogen in vivo. Differentially expressed genes identified by comparison of in vitro and in vivo conditions provided an insight into the physiological process of RA infection and provided an opportunity to identify additional virulence factors.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Óperon
/
Fosfotransferases
/
Virulência
/
Técnicas In Vitro
/
RNA Bacteriano
/
Transdução de Sinais
/
Genes vif
/
Análise de Sequência de RNA
/
Fatores de Virulência
/
Patos
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2018
Tipo de documento:
Artigo
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