The degree of major histocompatibility complex matching between purebred Maltese and mongrel dogs using microsatellite markers
Journal of Veterinary Science
;
: e5-2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-758902
ABSTRACT
Long-term maintenance of transplanted organs is one of the major factors that increases survival time of recipients. Although obtaining a major histocompatibility complex (MHC)-matched donor with the recipient is essential for successful organ transplantation, there have been limited reports on MHC matching between dogs. In this study, we analyzed the canine MHC matching rates using Maltese, one of the most popular purebred dogs, and mongrel dogs in Korea. Genomic DNA was extracted from blood leukocytes and DNA was amplified by polymerase chain reaction with primers specific to MHC microsatellite markers. The MHC matching degree was confirmed by the microsatellite markers using polyacrylamide gel electrophoresis. The MHC matching rates of each donor-recipient groups including Maltese-Maltese, mongrel-mongrel and Maltese-mongrel were 4.76%, 5.13% and 6.67%, respectively. There were no significant differences in the MHC matching degree between each group. These results demonstrate that MHC-matched donors could be selected from other breeds as much as from the same breed for transplantation. Knowledge of the MHC matching degree of purebred and mongrel dogs would offer valuable information not only for improving the success rate of organ transplantation surgery in canine patients but also for transplantation research using experimental canine models.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doadores de Tecidos
/
DNA
/
Reação em Cadeia da Polimerase
/
Transplante de Órgãos
/
Repetições de Microssatélites
/
Transplantes
/
Eletroforese em Gel de Poliacrilamida
/
Coreia (Geográfico)
/
Leucócitos
/
Complexo Principal de Histocompatibilidade
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2019
Tipo de documento:
Artigo
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