Histopathological and microbiological findings in buffalo chronic mastitis: evidence of tertiary lymphoid structures
Journal of Veterinary Science
;
: e28-2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-758910
ABSTRACT
Buffalo mastitis is an important economic problem in southern Italy, causing qualitative/quantitative alterations in milk and resulting in economic losses due to the sub-clinical course and chronic evolution. We investigated 50 udders of slaughtered buffaloes and subjected them to effectual microbiological screening to evaluate macro and microscopic mammary gland changes, immune-characterize the cell infiltrates, and compare the degree of tissue inflammation with somatic cell counts. Numerous Gram-positive and Gram-negative bacteria were isolated from all samples, majority of which were environmental mastitis pathogens. Histological features referable to chronic mastitis were observed in 92% udders. Lymphocytes, plasma cells and macrophages were found to evolve into aggregates in 48% udders, which often organized to form tertiary lymphoid structures (TLSs). A predominance of interstitial CD8+ over CD4+ lymphocytes and, in TLSs, scattered CD8+ lymphocytes in the mantle cells and CD79+ lymphocytes in germinal centers, were evidenced. Environmental pathogens are known to persist and cause chronic inflammatory changes in buffaloes, where CD8+ lymphocytes play an important role by controlling the local immune response. Moreover, the TLSs evidenced here for the first time in buffalo mastitis, could play a role in maintaining immune responses against persistent antigens, thereby contributing in determining the chronic course of mastitis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmócitos
/
Búfalos
/
Linfócitos
/
Contagem de Células
/
Programas de Rastreamento
/
Centro Germinativo
/
Glândulas Mamárias Humanas
/
Leite
/
Bactérias Gram-Negativas
/
Inflamação
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Pesquisa qualitativa
/
Estudo de rastreamento
Limite:
Animais
País/Região como assunto:
Europa
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2019
Tipo de documento:
Artigo
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