Autologous hybrid cell fusion vaccine in a spontaneous intermediate model of breast carcinoma
Journal of Veterinary Science
;
: e48-2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-758943
ABSTRACT
Breast cancer is among the most common malignancies affecting women and reproductively intact female dogs, resulting in death from metastatic disease if not treated effectively. To better manage the disease progression, canine mammary tumor (CMT) cells derived from malignant canine mammary cancers were fused to autologous dendritic cells (DCs) to produce living hybrid-cell fusion vaccines for canine patients diagnosed with spontaneous mammary carcinoma. The high-speed sorting of rare autologous canine patient DCs from the peripheral blood provides the autologous component of fusion vaccines, and fusion to major histocompatibility complex-unmatched CMT cells were produced at high rates. The vaccinations were delivered to each patient following a surgical resection 3 times at 3-week intervals in combination with immuno-stimulatory oligonucleotides and Gemcitabine adjunct therapy. The immunized patient animals survived 3.3-times longer (median survival 611 days) than the control patients (median survival 184 days) and also appeared to exhibit an enhanced quality of life. A comparison of vaccinated patients diagnosed with inflammatory mammary carcinoma resulted in a very short median survival (42 days), suggesting no effect of vaccination. The data showed that the development of autologous living DC-based vaccine strategies in patient animals designed to improve the management of canine mammary carcinoma can be successful and may allow an identification of the antigens that can be translatable to promote effective immunity in canine and human patients.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligonucleotídeos
/
Qualidade de Vida
/
Células Dendríticas
/
Mama
/
Neoplasias da Mama
/
Vacinas
/
Vacinação
/
Progressão da Doença
/
Histocompatibilidade
/
Células Híbridas
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2019
Tipo de documento:
Artigo
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