Performance Evaluation of Two Immunochromatographic Assays for Rotavirus Detection in Stool Specimens
Laboratory Medicine Online
;
: 73-76, 2019.
Artigo
em Coreano
| WPRIM
| ID: wpr-760488
ABSTRACT
BACKGROUND:
Rotavirus is a major pathogen causing enteritis worldwide in children under five years of age. In recent years, immunochromatographic assay (ICA) has been widely used as a diagnostic test for rotavirus detection. This study aimed to compare and evaluate the performance of ICA-based rotavirus rapid test kits from two manufacturers.METHODS:
Residual stool samples from a total of 130 children with acute enterocolitis from November 2017 to January 2018 were used. We compared the results of the two immunochromatographic methods (SD BIOLINE Rotavirus kit and GENEDIA Rotavirus Ag Rapid Test) with those of the currently used enzyme immunoassay method.RESULTS:
Positive agreement, negative agreement, and total agreement rates between the SD BIOLINE rotavirus kit and the enzyme immunoassay were 98.0%, 100%, and 99.2%, respectively. Positive agreement, negative agreement, and total agreement rates between the GENEDIA Rotavirus Ag Rapid Test and the enzyme immunoassay were 96.0%, 100%, and 98.4%, respectively.CONCLUSIONS:
Both rotavirus rapid test kits showed very good agreement with the conventional enzyme immunoassay. Therefore, it could be a useful test to detect rotavirus directly from stool samples in a short time.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cromatografia de Afinidade
/
Técnicas Imunoenzimáticas
/
Rotavirus
/
Testes Diagnósticos de Rotina
/
Enterite
/
Enterocolite
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Criança
/
Humanos
Idioma:
Coreano
Revista:
Laboratory Medicine Online
Ano de publicação:
2019
Tipo de documento:
Artigo
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