RNA-sequencing Profiles of Cell Cycle–Related Genes Upregulated during the G2-Phase in Giardia lamblia
The Korean Journal of Parasitology
;
: 185-189, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-761721
ABSTRACT
To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the 2 stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. Transcriptome analysis of cells in 2 distinct cell cycle stages of G. lamblia confirmed previously reported components of cell cycle (PcnA, cyclin B, and CDK) and identified additional cell cycle components (NEKs, Mad2, spindle pole protein, and CDC14A). This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Nocodazol
/
Ciclo Celular
/
Giardia lamblia
/
Afidicolina
/
Genes cdc
/
Técnicas de Cultura de Células
/
Ciclina B
/
Perfilação da Expressão Gênica
/
Pontos de Checagem do Ciclo Celular
/
Polos do Fuso
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2019
Tipo de documento:
Artigo
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