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Increased expression of vascular endothelial growth factor-C and vascular endothelial growth factor receptor-3 after pilocarpine-induced status epilepticus in mice
The Korean Journal of Physiology and Pharmacology ; : 281-289, 2019.
Artigo em Inglês | WPRIM | ID: wpr-761788
ABSTRACT
Vascular endothelial growth factor (VEGF)-C and its receptor, vascular endothelial growth factor receptor (VEGFR)-3, are responsible for lymphangiogenesis in both embryos and adults. In epilepsy, the expression of VEGF-C and VEGFR-3 was significantly upregulated in the human brains affected with temporal lobe epilepsy. Moreover, pharmacologic inhibition of VEGF receptors after acute seizures could suppress the generation of spontaneous recurrent seizures, suggesting a critical role of VEGF-related signaling in epilepsy. Therefore, in the present study, the spatiotemporal expression of VEGF-C and VEGFR-3 against pilocarpine-induced status epilepticus (SE) was investigated in C57BL/6N mice using immunohistochemistry. At 1 day after SE, hippocampal astrocytes and microglia were activated. Pyramidal neuronal death was observed at 4 days after SE. In the subpyramidal zone, VEGF-C expression gradually increased and peaked at 7 days after SE, while VEGFR-3 was significantly upregulated at 4 days after SE and began to decrease at 7 days after SE. Most VEGF-C/VEGFR-3-expressing cells were pyramidal neurons, but VEGF-C was also observed in some astrocytes in sham-manipulated animals. However, at 4 days and 7 days after SE, both VEGFR-3 and VEGF-C immunoreactivities were observed mainly in astrocytes and in some microglia of the stratum radiatum and lacunosum-moleculare of the hippocampus, respectively. These data indicate that VEGF-C and VEGFR-3 can be upregulated in hippocampal astrocytes and microglia after pilocarpine-induced SE, providing basic information about VEGF-C and VEGFR-3 expression patterns following acute seizures.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Convulsões / Estado Epiléptico / Encéfalo / Imuno-Histoquímica / Astrócitos / Células Piramidais / Microglia / Receptores de Fatores de Crescimento do Endotélio Vascular / Receptor 3 de Fatores de Crescimento do Endotélio Vascular / Fator A de Crescimento do Endotélio Vascular Limite: Animais / Humanos Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2019 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Convulsões / Estado Epiléptico / Encéfalo / Imuno-Histoquímica / Astrócitos / Células Piramidais / Microglia / Receptores de Fatores de Crescimento do Endotélio Vascular / Receptor 3 de Fatores de Crescimento do Endotélio Vascular / Fator A de Crescimento do Endotélio Vascular Limite: Animais / Humanos Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2019 Tipo de documento: Artigo