Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 8-13, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-763350
ABSTRACT
OBJECTIVE:
Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal spermatozoa, DNA fragmentation level, and hyaluronic acid (HA) binding ability.METHODS:
Thirty men with a total motile spermatozoa count >80 million participated. Semen samples were divided into four aliquots, which were processed using PureSperm, PureCeption, Sidney, and SpermGrad media, respectively. The DNA fragmentation level was measured using the Halosperm assay kit and HA binding ability was measured using the HBA assay kit.RESULTS:
The mean percentage of morphologically normal spermatozoa was significantly enhanced after DGC using all four media (10.3%, 9.9%, 9.8%, and 10.7%, respectively; p0.05). HA binding ability did not change after DGC using any of the four media.CONCLUSION:
The four media were equally effective for obtaining a sperm fraction with highly motile, morphologically normal sperm. PureSperm, PureCeption, and SpermGrad media were equally effective for acquiring a sperm fraction with less DNA fragmentation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sêmen
/
Espermatozoides
/
DNA
/
Centrifugação com Gradiente de Concentração
/
Fragmentação do DNA
/
Ácido Hialurônico
Limite:
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Clinical and Experimental Reproductive Medicine
Ano de publicação:
2019
Tipo de documento:
Artigo
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