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Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor
Endocrinology and Metabolism ; : 291-301, 2019.
Artigo em Inglês | WPRIM | ID: wpr-763711
ABSTRACT

BACKGROUND:

Striatin and caveolin-1 (cav-1) are scaffolding/regulating proteins that are associated with salt-sensitive high blood pressure and promote renal sodium and water reabsorption, respectively. The mineralocorticoid receptor (MR) interacts with striatin and cav-1, while aldosterone increases striatin and cav-1 levels. However, no in vivo data have been reported for the levels of these proteins in the kidney.

METHODS:

Male Wistar rats were intraperitoneally injected with normal saline solution, aldosterone alone (Aldo 150 µg/kg body weight), or aldosterone after pretreatment with eplerenone, an MR blocker, 30 minutes before the aldosterone injection (eplerenone [Ep.]+Aldo). Thirty minutes after the aldosterone injection, the amount and localization of striatin and cav-1 were determined by Western blot analysis and immunohistochemistry, respectively.

RESULTS:

Aldosterone increased striatin levels by 150% (P<0.05), and cav-1 levels by 200% (P<0.001). Eplerenone had no significant effect on striatin levels, but partially blocked the aldosterone-induced increase in cav-1 levels. Aldosterone stimulated striatin and cav-1 immunoreactivity in both the cortex and medulla. Eplerenone reduced cav-1 immunostaining in both areas; however, striatin intensity was reduced in the cortex, but increased in the medulla.

CONCLUSION:

This is the first in vivo study demonstrating that aldosterone rapidly enhances renal levels of striatin and cav-1. Aldosterone increases striatin levels via an MR-independent pathway, whereas cav-1 is partially regulated through MR.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Sódio / Imuno-Histoquímica / Água / Cloreto de Sódio / Western Blotting / Ratos Wistar / Receptores de Mineralocorticoides / Aldosterona / Caveolina 1 / Hipertensão Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Endocrinology and Metabolism Ano de publicação: 2019 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Sódio / Imuno-Histoquímica / Água / Cloreto de Sódio / Western Blotting / Ratos Wistar / Receptores de Mineralocorticoides / Aldosterona / Caveolina 1 / Hipertensão Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Endocrinology and Metabolism Ano de publicação: 2019 Tipo de documento: Artigo