Aldosterone Rapidly Enhances Levels of the Striatin and Caveolin-1 Proteins in Rat Kidney: The Role of the Mineralocorticoid Receptor
Endocrinology and Metabolism
;
: 291-301, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-763711
ABSTRACT
BACKGROUND:
Striatin and caveolin-1 (cav-1) are scaffolding/regulating proteins that are associated with salt-sensitive high blood pressure and promote renal sodium and water reabsorption, respectively. The mineralocorticoid receptor (MR) interacts with striatin and cav-1, while aldosterone increases striatin and cav-1 levels. However, no in vivo data have been reported for the levels of these proteins in the kidney.METHODS:
Male Wistar rats were intraperitoneally injected with normal saline solution, aldosterone alone (Aldo 150 µg/kg body weight), or aldosterone after pretreatment with eplerenone, an MR blocker, 30 minutes before the aldosterone injection (eplerenone [Ep.]+Aldo). Thirty minutes after the aldosterone injection, the amount and localization of striatin and cav-1 were determined by Western blot analysis and immunohistochemistry, respectively.RESULTS:
Aldosterone increased striatin levels by 150% (P<0.05), and cav-1 levels by 200% (P<0.001). Eplerenone had no significant effect on striatin levels, but partially blocked the aldosterone-induced increase in cav-1 levels. Aldosterone stimulated striatin and cav-1 immunoreactivity in both the cortex and medulla. Eplerenone reduced cav-1 immunostaining in both areas; however, striatin intensity was reduced in the cortex, but increased in the medulla.CONCLUSION:
This is the first in vivo study demonstrating that aldosterone rapidly enhances renal levels of striatin and cav-1. Aldosterone increases striatin levels via an MR-independent pathway, whereas cav-1 is partially regulated through MR.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sódio
/
Imuno-Histoquímica
/
Água
/
Cloreto de Sódio
/
Western Blotting
/
Ratos Wistar
/
Receptores de Mineralocorticoides
/
Aldosterona
/
Caveolina 1
/
Hipertensão
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Endocrinology and Metabolism
Ano de publicação:
2019
Tipo de documento:
Artigo
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