Changes in 11β-Hydroxysteroid Dehydrogenase and Glucocorticoid Receptor Expression in Kawasaki Disease
Korean Circulation Journal
;
: 377-382, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-76469
ABSTRACT
BACKGROUND AND OBJECTIVES:
This study aims to investigate the significance of changes in the expression 11β-hydroxysteroid dehydrogenase (11β-HSD) and glucocorticoid receptor (GR) for the development of Kawasaki disease (KD). SUBJECTS ANDMETHODS:
Real-time polymerase chain reaction was performed to determine the mRNA expression levels of GR and 11β-HSD in peripheral blood monocytes, both in the acute phase of the disease and after treatment. Western blotting was performed to determine the protein expression levels of GR and 11β-HSD.RESULTS:
The expression levels of GRβ, GRβ, and 11β-HSD1 mRNA in the acute phase were significantly higher than levels at baseline (p<0.01) and after treatment (p<0.05). The 11β-HSD2 mRNA levels were lower in the acute phase than in the normal group (p<0.01), and they were significantly higher after treatment than before (p<0.01). Western blot results were consistent with the real-time PCR results. The coronary artery lesion group exhibited significantly different 11β-HSD2 expression levels from that of the group with normal coronary arteries (p<0.01).CONCLUSION:
GR and 11β-HSD expression changes in the acute phase of KD are important factors for regulating inflammatory responses in KD.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxirredutases
/
RNA Mensageiro
/
Monócitos
/
Receptores de Glucocorticoides
/
Western Blotting
/
Vasos Coronários
/
Reação em Cadeia da Polimerase em Tempo Real
/
Síndrome de Linfonodos Mucocutâneos
Idioma:
Inglês
Revista:
Korean Circulation Journal
Ano de publicação:
2017
Tipo de documento:
Artigo
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