Bone Regeneration Using Block-type Deproteinized Porcine Bone Mineral with Collagen Membrane Using 3,4-Dihydroxyphenylalanine as Bone Adhesive
Journal of Korean Dental Science
;
: 43-56, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-764787
ABSTRACT
PURPOSE:
The purpose of this study was to assess the adhesiveness and cytotoxicity of 3, 4-dihydroxyphenylalanine (DOPA), and to evaluate the role of collagen membrane with DOPA in the guided bone regeneration. MATERIALS ANDMETHODS:
Peel resistance and cell cytotoxicity test were performed. Four defect types in nine rabbit calvaria were randomly allocated i) control, ii) membrane, iii) deproteinized porcine bone mineral (DPBM) covered by membrane with DOPA, and iv) DPBM covered by membrane with cyanoacrylate. Animals were sacrificed at 2 (n=4) and 8 weeks (n=5) for microcomputed tomography and histomorphometric analysis. DOPA showed low peel resistance but high cell viability.RESULT:
Cyanoacrylate and DOPA groups showed significantly higher mineralized tissue volume (MTV) compared to control and membrane groups at 2 weeks (P < 0.05). At 8 weeks, DOPA group showed the highest MTV. Significantly higher new bone area was found in DOPA group at 8 weeks (P < 0.05). Bone formation increased from 2 to 8 weeks in DOPA group (P < 0.05).CONCLUSION:
DOPA showed high cell viability and in vivo study revealed predictable performance in bone regeneration.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteogênese
/
Crânio
/
Regeneração Óssea
/
Di-Hidroxifenilalanina
/
Sobrevivência Celular
/
Adesividade
/
Adesivos
/
Colágeno
/
Cianoacrilatos
/
Microtomografia por Raio-X
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Korean Dental Science
Ano de publicação:
2018
Tipo de documento:
Artigo
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