Mitigation effects of red Platycodon grandiflorum extract on lipopolysaccharide-induced inflammation in splenocytes isolated from mice / 한국영양학회지
Journal of Nutrition and Health
;
: 243-249, 2019.
Artigo
em Coreano
| WPRIM
| ID: wpr-765986
ABSTRACT
PURPOSE:
Platycodon grandiflorum (PG) is known to have effective antimicrobial and anticancer activity. The main bioactive components of PG are saponins, and these could contribute to anti-inflammatory activity. However, little is known about the anti-inflammatory effect of PG. In this study, we aim to assess the anti-inflammatory response to Red PG Extract (RPGE) in splenocytes under ex vivo conditions.METHODS:
The cell viability of isolated splenocytes taken from mice was analyzed by performing a Cell Counting Kit-8 assay. The productions of nitric oxide (NO) and cytokines (specifically interleukin-6 (IL-6) and interleukin-10 (IL-10)) were measured utilizing Griess reagent and ELISA, respectively.RESULTS:
We found that co-treatment with RPGE and Lipopolysaccharide (LPS) decreased isolated splenocyte proliferation as compared with that of the LPS-stimulated control. We also observed that RPGE markedly suppressed NO synthesis and IL-6 production that was induced by LPS. There were no significant differences of IL-10 production between co-treatment with RPGE plus LPS and treatment with LPS alone.CONCLUSION:
When taken together, our data has shown that RPGE mitigates LPS-induced inflammation in splenocytes isolated from mice. Further research is surely needed to confirm the anti-inflammation effects of RPGE in an in vivo model.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Saponinas
/
Ensaio de Imunoadsorção Enzimática
/
Contagem de Células
/
Sobrevivência Celular
/
Citocinas
/
Interleucina-6
/
Interleucina-10
/
Platycodon
/
Inflamação
/
Óxido Nítrico
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of Nutrition and Health
Ano de publicação:
2019
Tipo de documento:
Artigo
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