Real-time PCR quantification of 9 periodontal pathogens in saliva samples from periodontally healthy Korean young adults
Journal of Periodontal & Implant Science
;
: 261-271, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-766065
ABSTRACT
PURPOSE:
Few studies have examined periodontal pathogens from saliva samples in periodontally healthy young adults. The purposes of this study were to determine the prevalence of periodontopathic bacteria and to quantify periodontal pathogens in saliva samples using real-time polymerase chain reaction (PCR) assays in periodontally healthy Korean young adults under 35 years of age.METHODS:
Nine major periodontal pathogens were analyzed by real-time PCR in saliva from 94 periodontally healthy young adults. Quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Campylobacter rectus, Peptostreptococcus anaerobius, and Eikenella corrodens was performed by DNA copy number measurement.RESULTS:
F. nucleatum and E. corrodens were detected in all subjects; the numbers of positive samples were 87 (92.6%), 91 (96.8%), and 90 (95.7%) for P. gingivalis, P. anaerobius, and C. rectus, respectively. Other pathogens were also detected in periodontally healthy subjects. Analysis of DNA copy numbers revealed that the most abundant periodontal pathogen was F. nucleatum, which was significantly more prevalent than all other bacteria (P < 0.001), followed by P. anaerobius, P. gingivalis, E. corrodens, C. rectus, and T. denticola. There was no significant difference in the prevalence of each bacterium between men and women. The DNA copy number of total bacteria was significantly higher in men than in women.CONCLUSIONS:
Major periodontal pathogens were prevalent in the saliva of periodontally healthy Korean young adults. Therefore, we suggest that the development of periodontal disease should not be overlooked in periodontally healthy young people, as it can arise due to periodontal pathogen imbalance and host susceptibility.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptostreptococcus
/
Doenças Periodontais
/
Saliva
/
Bactérias
/
DNA
/
Prevalência
/
Eikenella corrodens
/
Fusobacterium nucleatum
/
Aggregatibacter actinomycetemcomitans
/
Porphyromonas gingivalis
Tipo de estudo:
Estudo de prevalência
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Journal of Periodontal & Implant Science
Ano de publicação:
2018
Tipo de documento:
Artigo
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