Expression of miR-454-3p and its effect on proliferation, invasion and metastasis of colon cancer / 南方医科大学学报
Journal of Southern Medical University
; (12): 1421-1426, 2018.
Article
em Zh
| WPRIM
| ID: wpr-771458
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To study the expression of miR-454-3p in colon cancer and its effect on colon cancer proliferation, invasion and hepatic metastasis.@*METHODS@#Specimens of tumor tissues and paired adjacent tissues were collected from 20 patients with colorectal cancer for detecting the expression levels of miR-454-3p using in situ hybridization. Colon cancer cell line SW480 was transfected with a lentiviral vector to induce miR-454-3p over-expression, and the changes in cell proliferation and invasion were observed using cell counting kit-8 (CCK-8), clone formation assay and Transwell experiment. The effect of miR- 454-3p over-expression on hepatic metastasis of colon cancer was assessed in BALB/c mouse models.@*RESULTS@#The results of in situ hybridization showed a significantly increased expression level of miR-454-3p in colon cancer tissues as compared with normal colon tissues ( < 0.05). In SW480 cells, over-expression of miR-454-3p significantly promoted the cell proliferation and invasion ( < 0.05). In BALB/c mice, SW480 cells over-expressing miR-454-3p showed a significantly higher potential for liver metastases than the control cells ( < 0.05).@*CONCLUSIONS@#miR-454-3p is overexpressed in the tumor tissues in patients with colorectal cancer and participates in the progression of colorectal cancer by promoting cancer cell proliferation, invasion, and liver metastasis. miR-454-3p may serve as a novel biomarker for colorectal cancer diagnosis and prognostic evaluation.
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WPRIM
Assunto principal:
Patologia
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Sincalida
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Biomarcadores Tumorais
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Movimento Celular
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Colo
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Neoplasias do Colo
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Progressão da Doença
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MicroRNAs
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Linhagem Celular Tumoral
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Proliferação de Células
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2018
Tipo de documento:
Article