Protein engineering: from directed evolution to computational design / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1843-1856, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-771748
ABSTRACT
By constructing mutant libraries and utilizing high-throughput screening methods, directed evolution has emerged as the most popular strategy for protein design nowadays. In the past decade, taking advantages of computer performance and algorithms, computer-assisted protein design has rapidly developed and become a powerful method of protein engineering. Based on the simulation of protein structure and calculation of energy function, computational design can alter the substrate specificity and improve the thermostability of enzymes, as well as de novo design of artificial enzymes with expected functions. Recently, machine learning and other artificial intelligence technologies have also been applied to computational protein engineering, resulting in a series of remarkable applications. Along the lines of protein engineering, this paper reviews the progress and applications of computer-assisted protein design, and current trends and outlooks of the development.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Especificidade por Substrato
/
Engenharia de Proteínas
/
Proteínas
/
Química
/
Evolução Molecular Direcionada
/
Ensaios de Triagem em Larga Escala
/
Genética
/
Metabolismo
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2019
Tipo de documento:
Artigo
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