Imprinting genes modified parthenogenetic embryonic stem cells produce full-term mouse via tetraploid complementation / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 910-918, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-771835
ABSTRACT
Parthenogenetic embryonic stem cells (pESCs) derived from bi-maternal genomes do not have competency of tetraploid complementation, due to lacking of paternal imprinting genes. To make pESCs possess fully development potentials and similar pluripotency to zygote-derived ESCs, we knocked out one allelic gene of the two essential maternal imprinting genes (H19 and IG) in their differentially methylated regions (DMR) via CRISPR/Cas9 system and obtained double knock out (DKO) pESCs. Maternal pESCs had similar morphology, expression levels of pluripotent makers and in vitro neural differentiation potentials to zygotes-derived ESCs. Besides that, DKO pESCs could contribute to full-term fetuses through tetraploid complementation, proving that they held fully development potentials. Derivation of DKO pESCs provided a type of major histocompatibility complex (MHC) matched pluripotent stem cells, which would benefit research in regenerative medicine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Partenogênese
/
Impressão Genômica
/
Células-Tronco Pluripotentes
/
Medicina Regenerativa
/
Células-Tronco Embrionárias
/
Técnicas de Inativação de Genes
/
Tetraploidia
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2019
Tipo de documento:
Artigo
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