Evaluation of the Protective Efficacy of a Fused OmpK/Omp22 Protein Vaccine Candidate against Acinetobacter baumannii Infection in Mice / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 155-158, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-772249
ABSTRACT
Acinetobacter baumannii (A. Baumannii) is an emerging opportunistic pathogen responsible for hospital-acquired infections, and which now constitutes a sufficiently serious threat to public health to necessitate the development of an effective vaccine. In this study, a recombinant fused protein named OmpK/Omp22 and two individual proteins OmpK and Omp22 were obtained using recombinant expression and Ni-affinity purification. Groups of BALB/c mice were immunized with these proteins and challenged with a clinically isolated strain of A. baumannii. The bacterial load in the blood, pathological changes in the lung tissue and survival rates after challenge were evaluated. Mice immunized with OmpK/Omp22 fused protein provided significantly greater protection against A. baumannii challenge than those immunized with either of the two proteins individually. The results provide novel clues for future design of vaccines against A. baumannii.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Proteínas da Membrana Bacteriana Externa
/
Sangue
/
Proteínas Recombinantes de Fusão
/
Infecções por Acinetobacter
/
Vacinas Bacterianas
/
Pneumonia Bacteriana
/
Acinetobacter baumannii
/
Modelos Animais de Doenças
/
Alergia e Imunologia
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2018
Tipo de documento:
Artigo
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