Filamentation initiated by Cas2 and its association with the acquisition process in cells / 国际口腔科学杂志·英文版
International Journal of Oral Science
;
(4): 29-29, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-772253
ABSTRACT
Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive immunity. The process is critical for the ecology of the oral microflora and oral health. Although molecular mechanisms for spacer acquisition are known, it has never been established if this process is associated with the morphological changes of bacteria. In this study, we demonstrated a novel Cas2-induced filamentation phenotype in E. coli that was regulated by co-expression of the Cas1 protein. A 30 amino acid motif at the carboxyl terminus of Cas2 is necessary for this function. By imaging analysis, we provided evidence to argue that Cas-induced filamentation is a step coupled with new spacer acquisition during which filaments are characterised by polyploidy with asymmetric cell division. This work may open new opportunities to investigate the adaptive immune response and microbial balance for oral health.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Inglês
Revista:
International Journal of Oral Science
Ano de publicação:
2019
Tipo de documento:
Artigo
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