Transient receptor potential Vanilloid 1-based gene therapy alleviates orthodontic pain in rats / 国际口腔科学杂志·英文版
International Journal of Oral Science
;
(4): 11-11, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-772272
ABSTRACT
Orthodontic pain that is induced by tooth movement is an important sequela of orthodontic treatment and has a significant effect on patient quality of life. Studies have shown that the high expression of transient receptor potential vanilloid 1 (TRPV1) in trigeminal ganglions plays a vital role in the transmission and modulation of orofacial pain. However, little is known about the role of TRPV1 in orthodontic pain. In this study, male Sprague-Dawley rats were randomly assigned to six groups to study the role of TRPV1 in the modulation of tooth-movement pain. The expression levels of TRPV1 mRNA and protein were determined by real-time PCR and western blot, respectively. Moreover, pain levels were assessed using the rat grimace scale (RGS). The role of TRPV1 in modulating tooth-movement pain was examined by injecting a TRPV1 antagonist into the trigeminal ganglia of rats. A lentivirus containing a TRPV1 shRNA sequence was constructed and transduced into the rats' trigeminal ganglia. The results showed that the expression levels of TRPV1 protein and mRNA were elevated following tooth-movement pain. Pain levels increased rapidly on the 1 day, peaked on the 3 day and returned to baseline on the 14 day. The TRPV1 antagonist significantly reduced tooth-movement pain. The lentivirus containing a TRPV1 shRNA sequence was able to inhibit the expression of TRPV1 and relieved tooth-movement pain. In conclusion, TRPV1-based gene therapy may be a treatment strategy for the relief of orthodontic pain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dor
/
Qualidade de Vida
/
Técnicas de Movimentação Dentária
/
Terapia Genética
/
Distribuição Aleatória
/
Peptídeo Relacionado com Gene de Calcitonina
/
Gânglio Trigeminal
/
Ratos Sprague-Dawley
/
Canais de Cátion TRPV
/
Dente Molar
Limite:
Animais
Idioma:
Inglês
Revista:
International Journal of Oral Science
Ano de publicação:
2019
Tipo de documento:
Artigo
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