Application of PeriCam PSI blood flow imaging perfusion system in rat brain ischemia reperfusion injury models / 浙江大学学报·医学版

ABSTRACT

OBJECTIVE@# To study the feasibility and effect of PeriCam PSI system guiding the establishment of ischemia/reperfusion injury model in rats.@*METHODS@# A total of 70 adult male Sprague-Dawley rats were divided into the control group(=6), PSI monitoring group(=34) and traditional operation group(=30). Ischemia reperfusion model was established with reference to improve Zea-Longa line plug method. After the model established, the blood flow to the brain of control group, PSI monitoring group (ischemic 2 h, 24 h reperfusion) were observed and recorded respectively with PSI. The rats were then executed after 24 h, and the 2,3,5-triphenyltetrazolium chloride (TTC) staining and HE staining were used to observe the brain tissue.@*RESULTS@# The survival rate and modeling success rate of PSI monitoring group were higher than those of the traditional operation group(all <0.05). The blood perfusion in the brain and the distribution of blood vessels were clearly observed in the control group, and the data were normal. In 2 h ischemic group, the arterial flow was interrupted in the right cerebral artery, and the blood flow in the middle arterial blood supply was significantly decreased than that in the control group(<0.05). After the recovery of 24 h, the artery in the right side of the brain was restored to blood flow, but the blood flow in the partial supply area decreased, unable to recover to normal level. The TTC staining results indicated that there were obvious infarcts in the right brain tissue of PSI monitoring group,and the infarct area was more stable than that of the traditional operation group. The results of HE staining showed that the structure of brain tissue in the control group was normal, and the morphological rules of nerve cells were not change. While in brain tissue from PSI monitoring group, cortex and ischemia half dark stripe, nerve cell degeneration, necrosis and glial fiber disintegration, liquefaction, and light color, screen mesh in ischemic central area were observed.@*CONCLUSIONS@# PSI system can guide ischemia reperfusion model building and improve the success rate of the model.