Screening of Shuanghuanglian Injection allergenic ingredients based on immune fingerprint / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1588-1595, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-774518
ABSTRACT
In this paper,immune fingerprint was used to screen the allergenic components of Shuanghuanglian Injection(SHLI) by enzyme-linked immuno sorbent assay(ELISA) combined with HPLC/MS method. ELISA-embedded anti-IgE antibody could successfully adsorb allergens in SHLI and its plasma samples containing drugs through different routes of administration,suggesting that SHLI can induce type I hypersensitivity in rats. HPLC fingerprints and MS map of SHLI and drug-containing plasma samples from different routes of administration before and after anti-IgE antibody adsorption were established. According to the similarity evaluation of HPLC fingerprints and analysis results MS map,the sensitization of traditional Chinese medicine injections can be changed by different administration methods. There were 22 kinds of components that can be adsorbed by specific anti-Ig E antibodies in Shuanghuanglian Injection and its drug-containing plasma,most of them were acids and nitrogen compounds. Based on supramolecular theory,it was inferred that these compounds came from SHLI or body,and may form supramolecular hapten,which results in immunotoxicity and allergic reaction when being used as injection instead of oral liquid. Immune fingerprint is not only used to screen out single component allergen,but also more comprehensive,sensitive and easy to operate. It can provide reference for the future research methods of allergic reaction of traditional Chinese medicine injections.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Medicamentos de Ervas Chinesas
/
Alérgenos
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Hipersensibilidade
/
Injeções
/
Medicina Tradicional Chinesa
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2019
Tipo de documento:
Artigo
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