Shenmai injection protects mitochondria from oxidative injury in myocardial cells and its mechanism / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 507-513, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-775286
ABSTRACT
OBJECTIVE@#To investigate the effect of Shenmai injection on myocardial cells with oxidative injury and the underlying mechanisms.@*METHODS@#Tert-butyl hydroperoxide (t-BHP) was used to induce the oxidative stress in H9c2 myocardial cells. The cell viability and ATP level were evaluated using MTT-colorimetric method and CellTiter-Glo luminescent cell viability assay. The oxygen respiration rate was examined by Clark oxygen electrode. Pyruvate and pyruvate dehydrogenase (PDH) levels were evaluated by ELISA kit. Western blot and quantitative real-time RT-PCR were employed to evaluate the expression of pyruvate dehydrogenase alpha 1(PDHA1) and pyruvate dehydrogenase kinase 1(PDK1).@*RESULTS@#Shenmai injection significantly improved viability and respiration of H9c2 myocardial cells after t-BHP injury (0.05).@*CONCLUSIONS@#Shenmai injection protects mitochondria from oxidative stress by increasing PDH level, which indicates that it may improve energy metabolism of myocardial cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Linhagem Celular
/
Sobrevivência Celular
/
Regulação da Expressão Gênica
/
Proteínas Serina-Treonina Quinases
/
Estresse Oxidativo
/
Piruvato Desidrogenase (Lipoamida)
/
Miócitos Cardíacos
/
Combinação de Medicamentos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2018
Tipo de documento:
Artigo
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