Rapamycin treatment starting at 24 h after cerebral ischemia/reperfusion exhibits protective effect on brain injury in rats / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 443-449, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-775295
ABSTRACT
OBJECTIVE@#To investigate whether rapamycin treatment starting at 24 h after cerebral ischemia/reperfusion(I/R) has protective effect on brain injury in rats.@*METHODS@#The rat I/R model was established by middle cerebral artery occlusion according to Longa's method. A total of 104 Sprague Dawley rats were randomly divided into sham group, model group, and rapamycin-treated groups (6 h or 24 h after modeling). Neurological function was assessed with neurological severity score (NSS). Triphenyl tetrazolium chloride (TTC) staining and Fluoro-Jade B (FJB) staining were used to examine the infarct volume and neuronal apoptosis, respectively. The expression of p-S6 protein in mTOR signaling pathway was detected by Western blot analysis.@*RESULTS@#Compared with sham group, NSS of the model group was significantly increased and TTC staining indicated obvious infarct area (all 0.05).@*CONCLUSIONS@#Rapamycin treatment starting at 24 h after I/R exhibits protective effect on brain injury in rats.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Traumatismo por Reperfusão
/
Distribuição Aleatória
/
Isquemia Encefálica
/
Resultado do Tratamento
/
Ratos Sprague-Dawley
/
Sirolimo
/
Infarto da Artéria Cerebral Média
/
Usos Terapêuticos
/
Tratamento Farmacológico
/
Imunossupressores
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2018
Tipo de documento:
Artigo
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