Expression of IL-17 in Bone Marrow of Patients with Multiple Myeloma and Its Effect in Pathogenesis / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1154-1158, 2019.
Article
em Zh
| WPRIM
| ID: wpr-775749
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the expression and pathogenesis of IL-17 in bone marrow blood of multiple myeloma (MM) patients.@*METHODS@#Expression levels of IL-6, TNF-α and IL-17 in bone marrow serum of 20 MM patients and 20 control subjects were detected by ELISA, and correlation analysis was performed to analyze the correlation IL-17 with IL-6 and TNF-α. The effect of IL-17 on the proliferation of MM cells treated with different concentration of IL-17 was detected by cell prollferation and toxicity tesis. The morphological changes of RAW264.7 cells treated with IL-17 were observed by tartrate resistant acid phosphatase (TRAP) staining.@*RESULTS@#The levels of IL-17, IL-6 and TNF- in the bone marrow of MM patients were all higher than those of the normal control group (P<0.05). The IL-17 level positively correlated with IL-6 and TNF-α levels (r=0.6045, P<0.01 and r=0.627, P<0.01). Cell proliferation and toxicity tests confirmed that IL-17 can promote the proliferation of multiple myeloma cells. TRAP staining revealed that IL-17 could induce differentiate of RAW264.7 cells into multinuclear giant cells.@*CONCLUSION@#IL-17 may be involved in the pathogenesis of MM and promotes the proliferation of tumor cells, and induces the activation of osteoclasts leading to increased bone destruction.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Osteoclastos
/
Medula Óssea
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Células da Medula Óssea
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Fator de Necrose Tumoral alfa
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Interleucina-17
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Mieloma Múltiplo
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2019
Tipo de documento:
Article