A Two-Step GRIN Lens Coating for In Vivo Brain Imaging / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 419-424, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-776486
ABSTRACT
The complex spatial and temporal organization of neural activity in the brain is important for information-processing that guides behavior. Hence, revealing the real-time neural dynamics in freely-moving animals is fundamental to elucidating brain function. Miniature fluorescence microscopes have been developed to fulfil this requirement. With the help of GRadient INdex (GRIN) lenses that relay optical images from deep brain regions to the surface, investigators can visualize neural activity during behavioral tasks in freely-moving animals. However, the application of GRIN lenses to deep brain imaging is severely limited by their availability. Here, we describe a protocol for GRIN lens coating that ensures successful long-term intravital imaging with commercially-available GRIN lenses.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Materiais Biocompatíveis
/
Encéfalo
/
Camundongos Transgênicos
/
Biologia Celular
/
Neuroimagem
/
Hipocampo
/
Lentes
/
Métodos
/
Camundongos Endogâmicos C57BL
Limite:
Animais
Idioma:
Inglês
Revista:
Neuroscience Bulletin
Ano de publicação:
2019
Tipo de documento:
Artigo
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