The effects of tectochrysin on prostate cancer cells apoptosis and its mechanism / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 283-288, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-776514
ABSTRACT
OBJECTIVE@#To investigate the effects of tectochrysin on prostate cancer cell line 22Rv.1 and reveal its molecular mechanism.@*METHODS@#Tectochrysin at the concentrations of 0~20 μg/ml was applied to 22Rv.1 cells and normal prostate cell RWPE-1. The proliferation activity of the cells was detected by MTS assay. Flow cytometry and hoechst 33342 staining were used to analyze the effects of drugs on cell apoptosis, death, cell cycle and nuclear type changes. LDH release test was used to analyze the cytotoxicity of the drug to 22Rv.1 cells. QPCR and Western blot were used to analyze the effects of the drug on the expressions of genes in 22Rv.1 cells. Finally, the tumor inhibited effect of the drug on the bearing tumor BALB/c mice were confirmed though anti-tumor experiment.@*RESULTS@#Tectochrysin could significantly inhibit the proliferation activity of 22Rv.1 cells and induced their apoptosis, and promoted the expressions of genes dr4, dr5, trail, p53, caspase-3, caspase-8, caspase-9, bid, bax and foxo3, inhibited the expressions of anti-apoptotic genes akt, pi3k and bcl-2.@*CONCLUSION@#Tectochrysin can induce prostate cancer cells apoptosis through affecting TRAIL and PI3K/AKT signaling pathways, and has anti-prostate cancer effect.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias da Próstata
/
Flavonoides
/
Transdução de Sinais
/
Apoptose
/
Linhagem Celular Tumoral
/
Tratamento Farmacológico
/
Ligante Indutor de Apoptose Relacionado a TNF
/
Metabolismo
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2019
Tipo de documento:
Artigo
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