Correlation study between accumulation of triterpenoids and expression of relative genes in Alisma orientale / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 942-947, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-777534
ABSTRACT
To research the correlation between accumulation of triterpenoids and expression of key enzymes genes in triterpenoid biosynthesis of Alisma orientale,the study utilized UPLC-MS/MS method to detect eight triterpenoids content in the tuber of A. orientale from different growth stages,including alisol A,alisol A 24 acetate,alisol B,alisol B 23 acetate,alisol C 23 acetate,alisol F,alisol F 24 acetate and alisol G,and then the Real time quantitative PCR was used to analyze the expression of key enzymes genes HMGR and FPPS in triterpenoid biosynthesis. Correlation analysis showed that there was a significant positive relation between the total growth of these eight triterpenoids and the average relative expression of HMGR and FPPS(HMGR r = 0. 998,P<0. 01; FPPS r = 0. 957,P<0. 05),respectively. Therefore,the study preliminarily determined that HMGR and FPPS genes could regulate the biosynthesis of triterpenoids in A. orientale,which laid a foundation for further research on the biosynthesis and regulation mechanism of triterpenoids in A. orientale.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas de Plantas
/
Triterpenos
/
Extratos Vegetais
/
Química
/
Cromatografia Líquida
/
Hidroximetilglutaril-CoA-Redutases NADP-Dependentes
/
Alisma
/
Tubérculos
/
Geraniltranstransferase
/
Espectrometria de Massas em Tandem
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2019
Tipo de documento:
Artigo
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