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Glutamine protects against oxidative stress injury through inhibiting the activation of PI3K/Akt signaling pathway in parkinsonian cell model
Environmental Health and Preventive Medicine ; : 4-4, 2019.
Artigo em Inglês | WPRIM | ID: wpr-777640
ABSTRACT
BACKGROUND@#Parkinson's disease is a neurodegenerative disorder, and recent studies suggested that oxidative stress contributes to the degeneration of dopamine cell in Parkinson's disease. Glutamine also has a positive role in reducing oxidative stress damage. In this study, we hypothesized that glutamine offers protection against oxidative stress injury in 1-methyl-4-phenylpyridinium (MPP)-induced Parkinson's disease cell model.@*METHODS@#MPP was used to induce PD models in PC12 cells and classified into control, M0 (MPP), G0 (glutamine), and M0+G0 groups. CCK-8 and AO/EB staining assays were used to examine cell proliferation and apoptosis, respectively. Western blotting was applied to examine the protein expression of PI3K, P-Akt, Akt, P-mTOR, and mTOR.@*RESULTS@#We showed that glutamine suppressed cytotoxicity induced by MPP in PC12 cells. MPP decreased the superoxide dismutase and glutathione peroxidase activity and increased the malondialdehyde content, which were restored by glutamine. Moreover, MPP increased the expression of PI3K, P-Akt, Akt, P-mTOR, and mTOR, which were inhibited by glutamine. And the antioxidant capacity of glutamine on PC12 cells could be improved by LY294002 and inhibited by IGF-1.@*CONCLUSION@#These results suggest that glutamine strengthens the antioxidant capacity in PC12 cells induced by MPP through inhibiting the activation of the PI3K/Akt signaling pathway. The effects of glutamine should be investigated and the protective mechanism of glutamine in PD must be explored in future studies.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Doença de Parkinson / Farmacologia / 1-Metil-4-fenilpiridínio / Análise de Variância / Estresse Oxidativo / Técnicas de Cultura de Células / Fosfatidilinositol 3-Quinases / Substâncias Protetoras / Modelos Animais de Doenças / Proteínas Proto-Oncogênicas c-akt Limite: Animais Idioma: Inglês Revista: Environmental Health and Preventive Medicine Ano de publicação: 2019 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Doença de Parkinson / Farmacologia / 1-Metil-4-fenilpiridínio / Análise de Variância / Estresse Oxidativo / Técnicas de Cultura de Células / Fosfatidilinositol 3-Quinases / Substâncias Protetoras / Modelos Animais de Doenças / Proteínas Proto-Oncogênicas c-akt Limite: Animais Idioma: Inglês Revista: Environmental Health and Preventive Medicine Ano de publicação: 2019 Tipo de documento: Artigo