A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study
Environmental Health and Preventive Medicine
;
: 40-40, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-777665
ABSTRACT
BACKGROUND@#Heat shock protein 70 (HSP70) exhibits protective effects against ultraviolet (UV)-induced premature skin aging. A standardized extract of Asparagus officinalis stem (EAS) is produced as a novel and unique functional food that induces HSP70 cellular expression. To elucidate the anti-photoaging potencies of EAS, we examined its effects on HSP70 expression levels in UV-B-irradiated normal human dermal fibroblasts (NHDFs).@*METHODS@#NHDFs were treated with 1 mg/mL of EAS or dextrin (vehicle control) prior to UV-B irradiation (20 mJ/cm). After culturing NHDFs for different time periods, HSP70 mRNA and protein levels were analyzed using real-time polymerase chain reaction and western blotting, respectively.@*RESULTS@#UV-B-irradiated NHDFs showed reduced HSP70 mRNA levels after 1-6 h of culture, which were recovered after 24 h of culture. Treatment with EAS alone for 24 h increased HSP70 mRNA levels in the NHDFs, but the increase was not reflected in its protein levels. On the other hand, pretreatment with EAS abolished the UV-B irradiation-induced reduction in HSP70 expression at both mRNA and protein levels. These results suggest that EAS is capable to preserve HSP70 quantity in UV-B-irradiated NHDFs.@*CONCLUSIONS@#EAS exhibits anti-photoaging potencies by preventing the reduction in HSP70 expression in UV-irradiated dermal fibroblasts.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Efeitos da Radiação
/
Pele
/
Raios Ultravioleta
/
Extratos Vegetais
/
Envelhecimento da Pele
/
Células Cultivadas
/
Reação em Cadeia da Polimerase
/
Telômero
/
Proteínas de Choque Térmico HSP70
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Environmental Health and Preventive Medicine
Ano de publicação:
2018
Tipo de documento:
Artigo
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