GC-MS separation and determination of 20 amino acids in biological samples based on ethyl chloroformate second derivation / 药学学报
Acta Pharmaceutica Sinica
;
(12): 615-619, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-779637
ABSTRACT
Due to the difference in structure and chemical properties of amino acids, it is difficult to determine amino acids with different properties by GC-MS. A method was established for the simultaneous determination of 20 amino acids by using ethyl chloroformate (ECF) as the derivatization reagent and adjusting pH 9-10 in the second derivatization in this study. The results showed that 20 amino acids were well separated and the compounds in the 2-20 μg·mL-1 concentrations were detected with a good linear response correlation coefficient r2 greater than 0.99. The results of precision and system adaptability show that RSDAstragalus mongolicus, mouse urine sample and mouse serum sample.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2017
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS