Molecular Identification of a Trichinella Isolate from a Naturally Infected Pig in Tibet, China
The Korean Journal of Parasitology
;
: 381-384, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-78168
ABSTRACT
The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from Tibet Trichinella isolate from a naturally infected pig. The Tibet Trichinella isolate was also identified by PCR amplification of the 5S ribosomal DNA intergenic spacer region (5S ISR) and mitochondrial large-subunit ribosomal RNA (mt-lsrDNA) gene sequences. The results showed that 2 DNA fragments (749 bp and 445 bp) of the Tibet Trichinella isolate were identical to that of the reference isolates of T. spiralis. The Tibet Trichinella isolate might be classifiable to T. spiralis. This is the first report on T. spiralis in southwestern China.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Doenças dos Suínos
/
Triquinelose
/
DNA Mitocondrial
/
DNA Ribossômico
/
RNA Ribossômico 5S
/
Trichinella spiralis
/
Análise de Sequência de DNA
/
Tibet
/
DNA de Helmintos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2011
Tipo de documento:
Artigo
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