The shear stress proteome of chondrocytes
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
;
: 9-15, 2005.
Artigo
em Coreano
| WPRIM
| ID: wpr-784605
ABSTRACT
screening the shear stress related proteins in chondrocytes using twodimensional electrophresis and MALDI-TOF. C-28/I2 cell line were grown The fluid-induced shear stress (FISS) was applied using a cone viscometer at a rotational velocity of 80rpm for periods of 12 hours. Control cultures were tested under identical conditions without mechanical load application. Collected samples were used for the two-dimensional electrophoresis and MALDI-TOF. The identified proteins were calcyclin, RPE-spondin, interleukin-2, extracellular signal regulated kinase (ERK), lamin B2, porA protein, and RET-ELE1 protein. All of them showed a decreased expression. In conclusion, seven proteins were identified as a shear stress related proteins in chondrocytes. As the destruction of articular cartilage is one of main pathogenesis of TMJ internal derangement, this study will give useful information for the understanding of the molecular aspect of TMJ disease.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfotransferases
/
Articulação Temporomandibular
/
Cartilagem Articular
/
Transtornos da Articulação Temporomandibular
/
Linhagem Celular
/
Programas de Rastreamento
/
Interleucina-2
/
Condrócitos
/
Proteoma
/
Eletroforese
Tipo de estudo:
Estudo prognóstico
/
Estudo de rastreamento
Idioma:
Coreano
Revista:
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
Ano de publicação:
2005
Tipo de documento:
Artigo
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