The Effect of ER:YAG Laser & ER,CR:YSGG Laser on the Tissue of the Inflammation-Induced Mouse

ABSTRACT

PURPOSE: This study was performed to find out the effects of the ErYAG laser (Key Laser) & Er,CrYSGG laser (Water Laser) on inflammatory tissues.MATERIALS AND METHODS: It was performed on about 20 g, 6 weeks male ICR mouses. They were grouped into the control (negative), the inflammation induced 'control' (positive), Er,CrYSGG laser exposured group after inducing inflammation, ErYAG lasere exposured group after inducing inflammation each 15 mouses. The mouses were applicated 0.5% DNFB 1 cc on ear skin twice a day for 4 days until symptom expression. After laser exposure, ear tissues were extracted and defined gene expression by RT-PCR. Then, tissue staining, lymphocytes observation, electromicroscophic laboratory were carried out.RESULTS: Interleukin-1beta was expressed much less in the A-laser exposed group. Interleukin-1beta & Tumor Necrosis Factor-alpha were expressed 7 times lesser in the A-laser exposed group. The number of Lymphocytes related to inflammation was decreased rapidly in the A-laser exposed group in vivo. he number of cavity recovered normal was a little bigger in the A-laser exposed group after 5 days.CONCLUSION: The expression of IL-1beta & TNF-alpha, hitologic change, observation with electron microscope shows that Erbium laser exposure causes lesser inflammation with A-laser rather than B-laser.