Anti-tumor Effects of Vascular Endothelial Growth Factor Receptor-3 Inhibitor on Oral Cancer Cells
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
;
: 239-245, 2012.
Artigo
em Coreano
| WPRIM
| ID: wpr-785159
ABSTRACT
PURPOSE: Vascular endothelial growth factor (VEGF) plays a key role in tumor angiogenesis and lymphangiogenesis including induction of endothelial cell proliferation, migration and capillary tube formation. E7080 (S1164, Selleck chemical, Houston, TX, USA) is a muti-targeted kinase inhibitor, which targets VEGF receptor-2, 3 (VEGFR-2, 3) and inhibits survival and proliferation of tumor cell. The purpose of this study was to determine the anti-tumor effect of E7080 on oral squamous cell carcinoma.METHODS:
An oral squamous cell carcinoma cell line, SCC-9 was used in this study. E7080 was applied to SCC-9 cells by 3 different concentrations (1, 5, 10 microg/mL). Control means no application of E7080. The cellular growth was evaluated by real-time cell electronic sensing and MTT assay. The signal transduction was evaluated by Western blotting.RESULTS:
In experimental group, SCC-9 cell proliferation was decreased and the VEGFR-3 downstream pathways were inhibited compared with control. Furthermore, increasing the concentration of E7080, the ability of E7080 to disturbance of SCC-9 cell proliferation was increased.CONCLUSION:
Proliferation of SCC-9 cells was inhibited by E7080, which was through by inhibition of VEGFR-3 downstream pathway. In vivo study with E7080 will be required to provide therapeutic benefits in oral squamous cell carcinoma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfotransferases
/
Capilares
/
Carcinoma de Células Escamosas
/
Transdução de Sinais
/
Linhagem Celular
/
Receptor 3 de Fatores de Crescimento do Endotélio Vascular
/
Células Endoteliais
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Fator A de Crescimento do Endotélio Vascular
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Linfangiogênese
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Proliferação de Células
Idioma:
Coreano
Revista:
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
Ano de publicação:
2012
Tipo de documento:
Artigo
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