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Docosahexaenoic Acid Inhibits Expression of Fibrotic Mediators in Mice With Chronic Pancreatitis
Journal of Cancer Prevention ; : 233-239, 2019.
Artigo em Inglês | WPRIM | ID: wpr-785914
ABSTRACT

BACKGROUND:

Chronic pancreatitis (CP) is an irreversible progressive disease that destroys exocrine parenchyma, which are replaced by fibrous tissue. As pancreatic fibrosis is a key feature of CP, reducing fibrotic protein content in the pancreas is crucial for preventing CP. Studies suggest that NF-κB facilitates the expression of fibrotic mediators in pancreas and protein kinase C-δ (PKC-δ) regulates NF-κB activation in stimulated pancreatic acinar cells. Docosahexaenoic acid (DHA) is an omega-3 fatty acid having anti-inflammatory and anti-fibrotic effects. It has been shown to inhibit NF-κB activity in cerulein-stimulated pancreatic acinar cells which is a cellular model of CP. In the present study, we investigated if DHA inhibits expression of fibrotic mediators by reducing PKC-δ and NF-κB expression in mouse pancreatic tissues with CP.

METHODS:

For six weeks, mice were weekly induced for acute pancreatitis to develop CP. Furthermore, acute pancreatitis was induced by hourly intraperitoneal injections of cerulein (50 μg/kg × 7). Mice were administered DHA (10 μM) via drinking water before and after CP induction.

RESULTS:

Cerulein-induced pancreatic damages like decreased pancreatic weight/total body weight, leukocyte infiltration, necrosis of acinar cells, and vacuolization were found to be inhibited by DHA. Additionally, DHA inhibited cerulein-induced fibrotic mediators like alpha-smooth muscle actin and fibronectin in pancreas. DHA reduced expression of PKC-δ and NF-κB p65 in pancreatic tissues of cerulein-treated mice.

CONCLUSIONS:

DHA may be beneficial in preventing CP by suppressing pancreatic expression of fibrotic mediators.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Pâncreas / Pancreatite / Proteínas Quinases / Água Potável / Peso Corporal / Fibrose / Ceruletídeo / Actinas / Fibronectinas / Pancreatite Crônica Limite: Animais Idioma: Inglês Revista: Journal of Cancer Prevention Ano de publicação: 2019 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Pâncreas / Pancreatite / Proteínas Quinases / Água Potável / Peso Corporal / Fibrose / Ceruletídeo / Actinas / Fibronectinas / Pancreatite Crônica Limite: Animais Idioma: Inglês Revista: Journal of Cancer Prevention Ano de publicação: 2019 Tipo de documento: Artigo