Curcumin Attenuates Acrolein-induced COX-2 Expression and Prostaglandin Production in Human Umbilical Vein Endothelial Cells
Journal of Lipid and Atherosclerosis
;
: 184-194, 2020.
Artigo
em Inglês
| WPRIM
| ID: wpr-786072
ABSTRACT
OBJECTIVE:
Inflammation is crucial to limiting vascular disease. Previously we reported that acrolein, a known toxin in tobacco smoke, might play an important role in the progression of atherosclerosis via an inflammatory response involving cyclooxygenase-2 (COX-2) and prostaglandin production in human umbilical vein endothelial cells (HUVECs). Curcumin has been known to improve vascular function and have anti-inflammatory properties. In this study, we investigated whether curcumin prevents the induction of inflammatory response caused by acrolein.METHODS:
Anti-inflammatory effects of curcumin were examined in acrolein-stimulated HUVECs. Induction of proteins, mRNA, prostaglandin and reactive oxygen species (ROS) were measured using immunoblot analysis, real-time reverse-transcription polymerase chain reaction, enzyme-linked immunosorbent assay and flow cytometry, respectively.RESULTS:
Curcumin attenuates inflammatory response via inhibition of COX-2 expression and prostaglandin production in acrolein-induced human endothelial cells. This inhibition by curcumin results in the abolition of phosphorylation of protein kinase C, p38 mitogen-activated protein kinase, and cAMP response element-binding protein. Furthermore, curcumin suppresses the production of ROS and endoplasmic reticulum stress via phosphorylation of eukaryotic initiation factor-2α caused by acrolein.CONCLUSION:
These results suggest that curcumin might be a useful agent against endothelial dysfunction caused by acrolein-induced inflammatory response.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Proteínas Quinases
/
Fumaça
/
Nicotiana
/
Doenças Vasculares
/
Acroleína
/
Proteína Quinase C
/
RNA Mensageiro
/
Ensaio de Imunoadsorção Enzimática
/
Reação em Cadeia da Polimerase
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Lipid and Atherosclerosis
Ano de publicação:
2020
Tipo de documento:
Artigo
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