Anti-inflammatory and Immunosuppressive Effects of Panax notoginseng
Natural Product Sciences
;
: 317-325, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-786428
ABSTRACT
Here, we designed to examine the anti-inflammatory effects on RAW264.7 cells and the immunosuppressive effects by evaluating interleukin-2 (IL-2) production in Jurkat T cells using a MeOH extract of Panax notoginseng roots. The results showed that the MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC₅₀ value of 7.08 µg/mL) and displayed effects on T cell activation at a concentration of 400 µg/mL. In efforts to identify the potent compounds, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active CH₂Cl₂-, EtOAc-, and butanol-soluble fractions led to the successful isolation and identification of eleven compounds, including two polyacetylenes (1, 2), a steroid saponin (3), seven dammarane-type ginsenosides (4 – 10), and an oleanane-type ginsenoside (11). Among them, compound 11 was isolated from this plant for the first time. Compound 2 exhibited potent inhibitory effects on NO synthesis and an immunosuppressive effect with IC₅₀ values of 2.28 and 65.57 µM, respectively.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas
/
Saponinas
/
Linfócitos T
/
Interleucina-2
/
Ginsenosídeos
/
Panax notoginseng
/
Polímero Poliacetilênico
/
Panax
/
Óxido Nítrico
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Natural Product Sciences
Ano de publicação:
2019
Tipo de documento:
Artigo
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