Analysis of chemical constituents of Moutan cortex by HPLC-QTOFMS / 药学实践杂志

ABSTRACT

Objective To analyze the main chemical constituents of Moutan cortex by high performance liquid chromatogra -phy-quadrupole-time of flight mass spectrometry (HPLC-QTOFMS).Methods A CAPCELL PAK C18 (3.0 mm ×100 mm, 3 μm) column was used to do chromatographic separation .0.1% aqueous formic acid solution and acetonitrile were used as mobile phase composition.Gradient elution was used with a flow rate of 0.5 ml/min;QTOFMS was applied for qualitative analysis under positive and negative ion mode .Results 48 major constituents of Moutan cortex were identified by QTOFMS and structure-relevant fragment ions under the optimized condition .Conclusion A simple and reliable method with the usage of HPLC-QTOFMS was established to identify the main chemical constituents of Moutan cortex .