Metabolic analysis of miconazole-treated Candida albicans by GC-MS / 药学实践杂志

ABSTRACT

Objective To investigate the metabolism in miconazole-treated Candida albicans ,search for possible biomar-kers and discuss the mechanism of miconazole .Methods GC-MS was employed to determine the metabolic fingerprint of Can-dida albicans before and after treatment with miconazole ,and the difference based on multivariate was compared by statistical analysis ,the potential biomarkers were screened out and the mechanism of miconazole was discussed .Results Twenty three metabolites was screened out as potential biomarkers ,and they were primarily involved in amino acid metabolism ,citrate cycle , glycolysis and lipid metabolism .Conclusion The antifungal activity of miconazole was played by affecting a variety of metabolic pathways .