Preparation of DNA-loaded chitosan nanoparticle vaccine / 药学实践杂志

ABSTRACT

Objective To study and optimize the preparation condition of pVAX1‐wapA‐loaded nanoparticles and deter‐mine the transfection efficiency .Methods The related effects of the crucial factors for the formation of nanoparticlesconcen‐tration of chitosan and TPP ,pH value ,N/P ratio were studied by single‐factor experiment ,with nanoparticles size and zeta potential as index .Cell transfection test was carried out to indicate that enhancement of cell transfection efficiency of nano‐car‐rier .Results Nanoparticles loaded DNA vaccine were nearly spherical shape with uniform particle size chitosan nanoparticle (CS) ,(219 .2 ± 18 .2) nm ;quaternary ammonium chitosan nanoparticles(CSTM) ,(222 .5 ± 15 .6) nm .Zeta potential of CS and CSTM was (24 .7 ± 3 .5) mV ,(19 .6 ± 1 .2) mV and encapsulation efficiency was 91 .24% ,87 .66% ,respectively .CSTM nano‐particle could enhance cellular uptake of pVAX1‐wapA obviously .Conclusion CSTM nanoparticle was proved to be an efficient DNA vaccine delivery vector .