Studies on the chemical constituents from marine sponge Agelas sp / 药学实践杂志

ABSTRACT

Objective To investigate the chemical constituents from marine sponge Agelas sp.Methods Multi-chromatographic methods including silica gel column chromatography, Sephadex-LH20 gel permeation and HPLC were employed for the isolation and purification.The structures were identified on the basis of chemical evidence and NMR spectra data.Results Nine compounds were isolated and identified as 4,5-dibromo-1H-pyrrole-2-carboxylic acid amide (1), 4,5-dibromo-1H-pyrrole-2-carboxylic acid (2), 4,5-dibromo-1H-pyrrole-2-carboxylic acid methyl ester (3), N-(4,5-dibromopyrrole-2-carbonyl) glycine methyl ester (4), 3-{[1-(4,5-dibromopyrrole-2-yl)-methanoyl]-amino}-propionic acid methyl ester (5), 3-{[1-(4,5-dibromopyrrole-2-yl)-methanoyl]-amino}-propionic acid ethyl ester (6),isoindole-1,3-dione (7), 2 (3H)-benzothiazolone (8), and manzacidin C (9).Conclusion Compounds 4～9 were isolated from the sponge of Agelas sp.for the first time,and compounds 4～6 were new natural products.